ABSTRACT
The aim of this cross-sectional study was to examine the relationship between the composition of the subgingival microbiota and the vitamin D receptor (VDR) gene polymorphism in Brazilian adults with chronic periodontitis. The clinical parameters of probing depth, clinical attachment level, bleeding on probing, plaque accumulation and suppuration were measured in 60 Caucasian adults who were divided into two groups: 30 healthy individuals (control) and 30 with chronic periodontitis (ChP). Subgingival plaque samples were collected from 6 sites per subject and analyzed for 38 bacterial species using the Checkerboard DNA-DNA Hybridization. DNA was obtained from the subjects' epithelial cells by scraping the buccal mucosa and using a mouthwash containing 3 percent of glucose. Polymorphism in the VDR gene was analyzed by the polymerase chain reaction (PCR), followed by Taql digestion (RFLP). The healthy subjects presented significantly lower levels (0.3 × 10(7) ± 0.7 × 10(7)) of total microbial counts in comparison with subjects with chronic periodontitis (4.5 × 10(7) ± 2.9 × 10(7)). Regarding the occurrence of VDR polymorphism, it was observed that the Tt genotype was more prevalent in the Periodontitis group (60 percent) than in the Healthy group (30 percent), while the prevalences of the TT genotype were 23.3 percent and 53.3 percent, respectively (Chi-square test, p < 0.05). No difference was found in the composition of subgingival microbiota among the VDR genotypes evaluated for the Healthy and Periodontitis groups. In conclusion, the Tt genotype was associated with periodontal disease; however, no association with the subgingival microbiota was observed.
Subject(s)
Adult , Female , Humans , Male , Chronic Periodontitis/genetics , Chronic Periodontitis/microbiology , Gingiva/microbiology , Polymorphism, Genetic , Receptors, Calcitriol/genetics , Chi-Square Distribution , Cross-Sectional Studies , DNA Probes , Genotype , Polymerase Chain Reaction , Statistics, NonparametricABSTRACT
O objetivo deste estudo transversal foi avaliar a presença do complexo vermelho em indivíduos com periodontite crônica e indivíduos periodontalmente saudáveis. Foram selecionados 30 indivíduos, sendo 15 periodalmente saudáveis e 15 com periodontite crónica. Os parâmetros clínicos de profundidade de sondagem (PS), nível clínico de inserção (NCI), presença ou ausência de sangramento à sondagem, placa supragengival visível e supuração, foram avaliados em seis sítios por dente.Amostras de placa subgengival foram coletadas de nove sítios mésio-vestibulares, no grupo saudável, e de seis sítios com PS e NIC ≥5 mm e três sítios com PS e NIC ≤4 mm no grupo com periodontite crónica. As amostras foram avaliadas pelo teste Checkerboarad DNA-DNA Hybridizatíonpaïa a presença de Tannerela forsythia, Treponema dentícola e Porphyromonas gingivalis. Diferenças microbiológicas entre os dois grupos foram avaliadas por meio do teste U de MannWhitney. O grupo de indivíduos periodontalmente saudáveis apresentou níveis médios de contagem (x105±DP) de T. forsythia, T.denticola, fg/ng/Vã/fs